portant;="" white-space:="" orphans:="" 2;="" widows:="" background-color:="" rgb(255,="" 255,="" 255);="" font-variant-ligatures:="" font-variant-caps:="" -webkit-text-stroke-width:="" text-decoration-style:="" initial;="" text-decoration-color:="" initial;"="" style="white-space: normal; caret-color: rgb(51, 51, 51); color: rgb(51, 51, 51); font-family: "Microsoft YaHei", Arial, Helvetica, sans-serif;">植物基因組DNA 快速提?。?0 次）
portant;="" white-space:="" orphans:="" 2;="" widows:="" background-color:="" rgb(255,="" 255,="" 255);="" font-variant-ligatures:="" font-variant-caps:="" -webkit-text-stroke-width:="" text-decoration-style:="" initial;="" text-decoration-color:="" initial;"="" style="white-space: normal; caret-color: rgb(51, 51, 51); color: rgb(51, 51, 51); font-family: "Microsoft YaHei", Arial, Helvetica, sans-serif;">概述：
portant;="" white-space:="" orphans:="" 2;="" widows:="" background-color:="" rgb(255,="" 255,="" 255);="" font-variant-ligatures:="" font-variant-caps:="" -webkit-text-stroke-width:="" text-decoration-style:="" initial;="" text-decoration-color:="" initial;"="" style="white-space: normal; caret-color: rgb(51, 51, 51); color: rgb(51, 51, 51); font-family: "Microsoft YaHei", Arial, Helvetica, sans-serif;">本方法可用于植物細胞DNA 的快速提取，可提取107 細胞，其質(zhì)量能夠滿足各種分子生物學要求。

portant;="" white-space:="" orphans:="" 2;="" widows:="" background-color:="" rgb(255,="" 255,="" 255);="" font-variant-ligatures:="" font-variant-caps:="" -webkit-text-stroke-width:="" text-decoration-style:="" initial;="" text-decoration-color:="" initial;"="" style="white-space: normal; caret-color: rgb(51, 51, 51); color: rgb(51, 51, 51); font-family: "Microsoft YaHei", Arial, Helvetica, sans-serif;">組成：
portant;="" white-space:="" orphans:="" 2;="" widows:="" background-color:="" rgb(255,="" 255,="" 255);="" font-variant-ligatures:="" font-variant-caps:="" -webkit-text-stroke-width:="" text-decoration-style:="" initial;="" text-decoration-color:="" initial;"="" style="white-space: normal; caret-color: rgb(51, 51, 51); color: rgb(51, 51, 51); font-family: "Microsoft YaHei", Arial, Helvetica, sans-serif;">1．溶液A 1.2ml RNase A 10mg/ml。-20℃保存
portant;="" white-space:="" orphans:="" 2;="" widows:="" background-color:="" rgb(255,="" 255,="" 255);="" font-variant-ligatures:="" font-variant-caps:="" -webkit-text-stroke-width:="" text-decoration-style:="" initial;="" text-decoration-color:="" initial;"="" style="white-space: normal; caret-color: rgb(51, 51, 51); color: rgb(51, 51, 51); font-family: "Microsoft YaHei", Arial, Helvetica, sans-serif;">2．溶液B 60ml
portant;="" white-space:="" orphans:="" 2;="" widows:="" background-color:="" rgb(255,="" 255,="" 255);="" font-variant-ligatures:="" font-variant-caps:="" -webkit-text-stroke-width:="" text-decoration-style:="" initial;="" text-decoration-color:="" initial;"="" style="white-space: normal; caret-color: rgb(51, 51, 51); color: rgb(51, 51, 51); font-family: "Microsoft YaHei", Arial, Helvetica, sans-serif;">3．溶液C 180ml
portant;="" white-space:="" orphans:="" 2;="" widows:="" background-color:="" rgb(255,="" 255,="" 255);="" font-variant-ligatures:="" font-variant-caps:="" -webkit-text-stroke-width:="" text-decoration-style:="" initial;="" text-decoration-color:="" initial;"="" style="white-space: normal; caret-color: rgb(51, 51, 51); color: rgb(51, 51, 51); font-family: "Microsoft YaHei", Arial, Helvetica, sans-serif;">4．溶液D 3ml Resin 用時充分混勻
portant;="" white-space:="" orphans:="" 2;="" widows:="" background-color:="" rgb(255,="" 255,="" 255);="" font-variant-ligatures:="" font-variant-caps:="" -webkit-text-stroke-width:="" text-decoration-style:="" initial;="" text-decoration-color:="" initial;"="" style="white-space: normal; caret-color: rgb(51, 51, 51); color: rgb(51, 51, 51); font-family: "Microsoft YaHei", Arial, Helvetica, sans-serif;">5. 溶液E 50ml 洗液(用前加入75ml 無水/酒精,充分混勻)
portant;="" white-space:="" orphans:="" 2;="" widows:="" background-color:="" rgb(255,="" 255,="" 255);="" font-variant-ligatures:="" font-variant-caps:="" -webkit-text-stroke-width:="" text-decoration-style:="" initial;="" text-decoration-color:="" initial;"="" style="white-space: normal; caret-color: rgb(51, 51, 51); color: rgb(51, 51, 51); font-family: "Microsoft YaHei", Arial, Helvetica, sans-serif;">6．溶液F 25ml TE buffer

portant;="" white-space:="" orphans:="" 2;="" widows:="" background-color:="" rgb(255,="" 255,="" 255);="" font-variant-ligatures:="" font-variant-caps:="" -webkit-text-stroke-width:="" text-decoration-style:="" initial;="" text-decoration-color:="" initial;"="" style="white-space: normal; caret-color: rgb(51, 51, 51); color: rgb(51, 51, 51); font-family: "Microsoft YaHei", Arial, Helvetica, sans-serif;">步驟：
portant;="" white-space:="" orphans:="" 2;="" widows:="" background-color:="" rgb(255,="" 255,="" 255);="" font-variant-ligatures:="" font-variant-caps:="" -webkit-text-stroke-width:="" text-decoration-style:="" initial;="" text-decoration-color:="" initial;"="" style="white-space: normal; caret-color: rgb(51, 51, 51); color: rgb(51, 51, 51); font-family: "Microsoft YaHei", Arial, Helvetica, sans-serif;">1. 組織≤0.1g，液氮研磨，加入0.3ml 溶液B，反復混勻，室溫5min。
portant;="" white-space:="" orphans:="" 2;="" widows:="" background-color:="" rgb(255,="" 255,="" 255);="" font-variant-ligatures:="" font-variant-caps:="" -webkit-text-stroke-width:="" text-decoration-style:="" initial;="" text-decoration-color:="" initial;"="" style="white-space: normal; caret-color: rgb(51, 51, 51); color: rgb(51, 51, 51); font-family: "Microsoft YaHei", Arial, Helvetica, sans-serif;">2. 加入0.8ml 溶液C，溶液A 20μl，充分混勻，室溫放置20min。
portant;="" white-space:="" orphans:="" 2;="" widows:="" background-color:="" rgb(255,="" 255,="" 255);="" font-variant-ligatures:="" font-variant-caps:="" -webkit-text-stroke-width:="" text-decoration-style:="" initial;="" text-decoration-color:="" initial;"="" style="white-space: normal; caret-color: rgb(51, 51, 51); color: rgb(51, 51, 51); font-family: "Microsoft YaHei", Arial, Helvetica, sans-serif;">3. ≥8000rpm 離心3min。
portant;="" white-space:="" orphans:="" 2;="" widows:="" background-color:="" rgb(255,="" 255,="" 255);="" font-variant-ligatures:="" font-variant-caps:="" -webkit-text-stroke-width:="" text-decoration-style:="" initial;="" text-decoration-color:="" initial;"="" style="white-space: normal; caret-color: rgb(51, 51, 51); color: rgb(51, 51, 51); font-family: "Microsoft YaHei", Arial, Helvetica, sans-serif;">4. 取上清，加入50ul 溶液D，室溫放置5min，≥8000rpm 離心1min，棄上清。
portant;="" white-space:="" orphans:="" 2;="" widows:="" background-color:="" rgb(255,="" 255,="" 255);="" font-variant-ligatures:="" font-variant-caps:="" -webkit-text-stroke-width:="" text-decoration-style:="" initial;="" text-decoration-color:="" initial;"="" style="white-space: normal; caret-color: rgb(51, 51, 51); color: rgb(51, 51, 51); font-family: "Microsoft YaHei", Arial, Helvetica, sans-serif;">5. 加入800μl 溶液C，混勻，≥8000rpm 離心1min，棄上清。
portant;="" white-space:="" orphans:="" 2;="" widows:="" background-color:="" rgb(255,="" 255,="" 255);="" font-variant-ligatures:="" font-variant-caps:="" -webkit-text-stroke-width:="" text-decoration-style:="" initial;="" text-decoration-color:="" initial;"="" style="white-space: normal; caret-color: rgb(51, 51, 51); color: rgb(51, 51, 51); font-family: "Microsoft YaHei", Arial, Helvetica, sans-serif;">6. 加入1ml 溶液E，混勻，≥8000rpm 離心30-60s，棄上清。
portant;="" white-space:="" orphans:="" 2;="" widows:="" background-color:="" rgb(255,="" 255,="" 255);="" font-variant-ligatures:="" font-variant-caps:="" -webkit-text-stroke-width:="" text-decoration-style:="" initial;="" text-decoration-color:="" initial;"="" style="white-space: normal; caret-color: rgb(51, 51, 51); color: rgb(51, 51, 51); font-family: "Microsoft YaHei", Arial, Helvetica, sans-serif;">7. 重復步驟6。
portant;="" white-space:="" orphans:="" 2;="" widows:="" background-color:="" rgb(255,="" 255,="" 255);="" font-variant-ligatures:="" font-variant-caps:="" -webkit-text-stroke-width:="" text-decoration-style:="" initial;="" text-decoration-color:="" initial;"="" style="white-space: normal; caret-color: rgb(51, 51, 51); color: rgb(51, 51, 51); font-family: "Microsoft YaHei", Arial, Helvetica, sans-serif;">8. ≥12000rpm,離心30-60s，吸干上清，室溫敞開蓋晾干約5-10min。
portant;="" white-space:="" orphans:="" 2;="" widows:="" background-color:="" rgb(255,="" 255,="" 255);="" font-variant-ligatures:="" font-variant-caps:="" -webkit-text-stroke-width:="" text-decoration-style:="" initial;="" text-decoration-color:="" initial;"="" style="white-space: normal; caret-color: rgb(51, 51, 51); color: rgb(51, 51, 51); font-family: "Microsoft YaHei", Arial, Helvetica, sans-serif;">9. 取溶液F 200-300μl，混勻，40-50℃水浴3-5min。
portant;="" white-space:="" orphans:="" 2;="" widows:="" background-color:="" rgb(255,="" 255,="" 255);="" font-variant-ligatures:="" font-variant-caps:="" -webkit-text-stroke-width:="" text-decoration-style:="" initial;="" text-decoration-color:="" initial;"="" style="white-space: normal; caret-color: rgb(51, 51, 51); color: rgb(51, 51, 51); font-family: "Microsoft YaHei", Arial, Helvetica, sans-serif;">10. ≥12000rpm 離心30-60s，取上清，即為DNA。

portant;="" white-space:="" orphans:="" 2;="" widows:="" background-color:="" rgb(255,="" 255,="" 255);="" font-variant-ligatures:="" font-variant-caps:="" -webkit-text-stroke-width:="" text-decoration-style:="" initial;="" text-decoration-color:="" initial;"="" style="white-space: normal; caret-color: rgb(51, 51, 51); color: rgb(51, 51, 51); font-family: "Microsoft YaHei", Arial, Helvetica, sans-serif;">注：
portant;="" white-space:="" orphans:="" 2;="" widows:="" background-color:="" rgb(255,="" 255,="" 255);="" font-variant-ligatures:="" font-variant-caps:="" -webkit-text-stroke-width:="" text-decoration-style:="" initial;="" text-decoration-color:="" initial;"="" style="white-space: normal; caret-color: rgb(51, 51, 51); color: rgb(51, 51, 51); font-family: "Microsoft YaHei", Arial, Helvetica, sans-serif;">1. 組織若多，可適當加大溶液B 和溶液D 的用量，其它成份不變。
portant;="" white-space:="" orphans:="" 2;="" widows:="" background-color:="" rgb(255,="" 255,="" 255);="" font-variant-ligatures:="" font-variant-caps:="" -webkit-text-stroke-width:="" text-decoration-style:="" initial;="" text-decoration-color:="" initial;"="" style="white-space: normal; caret-color: rgb(51, 51, 51); color: rgb(51, 51, 51); font-family: "Microsoft YaHei", Arial, Helvetica, sans-serif;">2. 混勻一定要溫和，否則DNA 大分子將被打斷，而部分降解。
portant;="" white-space:="" orphans:="" 2;="" widows:="" background-color:="" rgb(255,="" 255,="" 255);="" font-variant-ligatures:="" font-variant-caps:="" -webkit-text-stroke-width:="" text-decoration-style:="" initial;="" text-decoration-color:="" initial;"="" style="white-space: normal; caret-color: rgb(51, 51, 51); color: rgb(51, 51, 51); font-family: "Microsoft YaHei", Arial, Helvetica, sans-serif;">3. 適用新鮮組織、嫩組織，盡可能去除葉脈、葉柄。
portant;="" white-space:="" orphans:="" 2;="" widows:="" background-color:="" rgb(255,="" 255,="" 255);="" font-variant-ligatures:="" font-variant-caps:="" -webkit-text-stroke-width:="" text-decoration-style:="" initial;="" text-decoration-color:="" initial;"="" style="white-space: normal; caret-color: rgb(51, 51, 51); color: rgb(51, 51, 51); font-family: "Microsoft YaHei", Arial, Helvetica, sans-serif;">4. 液氮研磨一定要保證組織在研磨過程中形如干粉狀。
portant;="" white-space:="" orphans:="" 2;="" widows:="" background-color:="" rgb(255,="" 255,="" 255);="" font-variant-ligatures:="" font-variant-caps:="" -webkit-text-stroke-width:="" text-decoration-style:="" initial;="" text-decoration-color:="" initial;"="" style="white-space: normal; caret-color: rgb(51, 51, 51); color: rgb(51, 51, 51); font-family: "Microsoft YaHei", Arial, Helvetica, sans-serif;">5. 用戶可依實驗需要，適當調(diào)整溶液F 加入量，溶液F 越少，DNA 濃度越高，但產(chǎn)量略有損失，若想大限度提高產(chǎn)量，可重復步驟9、10。兩次所得DNA 可合在一處。